鸟嘌呤核苷酸交换因子GEFT抗体

英文名称Rabbit Anti-GEFT  antibody
中文名称鸟嘌呤核苷酸交换因子GEFT抗体
别    名ARHGEF25; ARHGP_HUMAN; GEFT; p63RhoGEF; Guanine nucleotide exchange factor GEFT; p63RhoGEF ; RAC/CDC42 exchange factor; Rac/Cdc42/Rho exchange factor GEFT; Rho guanine nucleotide exchange factor 25; RhoA/RAC/CDC42 exchange factor; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT.  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow)
产品应用IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量64kDa
细胞定位细胞浆 细胞膜 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human GEFT: 251-340/580 
亚    型IgG
纯化方法affinity purified by Protein A
缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍GEFT is a 580 amino acid cytoplasmic protein that is highly expressed in excitable tissues such as brain, heart and muscle, and weakly expressed in small intestine, colon, liver, placenta and lung. GEFT may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. GEFT works as a guanine nucleotide exchange factor for the Rho family of small GTPases and links specifically to G alpha q/11-coupled receptors in Rho A activation. GEFT is an important regulator of processes involved in axon and dendrite formation. Involved in skeletal myogenesis, GEFT seems to be an exchange factor primarily for Rac 1 in neurons. Existing as two alternatively spliced variants, GEFT contains a DH (DBL-homology) domain and a PH domain.

Function:
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.

Subunit:
Interacts (via the DH domain) with BVES (via the C-terminus cytoplasmic tail) (By similarity). Interacts with activated GNAQ and GNA11. Interacts with RHOA, CDC42 and RAC1.

Subcellular Location:
Cell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.

Tissue Specificity:
Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.

SWISS:
Q86VW2

Gene ID:
115557